molecular identification of salmonella infantis isolated from backyard chickens and detection of their resistance genes by pcr

Authors

a. ghoddusi

b. nayeri fasaei

v. karimi

i. ashrafi tamai

z. moulana

abstract

this study aims at molecular identification of salmonella infantis isolated from backyard chickens and the detection of their antibiotic resistance genes. a total of 46 salmonella-suspected samples isolated from backyard chickens of northern iran were collected. serotyping was done by the traditional method and then confirmed by pcr. antimicrobial susceptibility of the isolates against 13 antimicrobial agents was determined by the standard disk diffusion method. there were 44 samples identified as salmonella. serotyping results showed that all 44 isolates belonged to serogroup c1 and serovar infantis. the most resistance observed was to tetracycline and doxycycline (100%), chloramphenicol (79%) and florfenicol (72%). the flor, cati, teta and tetg genes were used for the detection of florfenicol chloramphenicol and tetracycline resistance. in order to identify the phenotypic resistance in strains which showed resistance genes by pcr, colony pcr and culture on plates each containing antibiotic was performed simultaneously. all the salmonella infantis resistant to florfenicol and chloramphenicol harbored flor and cati. none of the salmonella resistant to tetracycline carried teta or tetg. the result of colony pcr and culture in antibiotic medium confirmed theresults of pcr and indicated phenotypic resistance in these samples.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Molecular identification of Salmonella Infantis isolated from backyard chickens and detection of their resistance genes by PCR

This study aims at molecular identification of Salmonella Infantis isolated from backyard chickens and the detection of their antibiotic resistance genes. A total of 46 Salmonella-suspected samples isolated from backyard chickens of northern Iran were collected. Serotyping was done by the traditional method and then confirmed by PCR. Antimicrobial susceptibility of the isolates against 13 antim...

full text

Molecular identification of Salmonella Infantis isolated from backyard chickens and detection of their resistance genesby PCR.

This study aims at molecular identification of Salmonella Infantis isolated from backyard chickens and the detection of their antibiotic resistance genes. A total of 46 Salmonella-suspected samples isolated from backyard chickens of northern Iran were collected. Serotyping was done by the traditional method and then confirmed by PCR. Antimicrobial susceptibility of the isolates against 13 antim...

full text

Characterization of the Salmonella Isolates from Backyard Chickens in North of Iran, by Serotyping, Multiplex PCR and Antibiotic Resistance Analysis

The present study was designed to investigate the prevalence of Salmonella species, their molecular characterization and antibiotic resistance in backyard chickens. A total of 1125 samples were collected from backyard chickens in two consequent samplings. In the first part, samples were included of 820 poor recently hatched chicks, hatching residuals, egg shells in the nest floor, cloacal swabs...

full text

Molecular Identification of Salmonella Strains Isolated from Livestock in Alborz Province and Their Serotyping

Background and Objective: Salmonellosis is an important infectious zoonotic disease that makes it even more significant to identify and control the causative strains. Molecular methods, especially polymerase chain reaction (PCR), for virulence genes can help to quickly and accurately identify Salmonella strains. Accordingly, the purpose of this study was molecular identification based on sivH, ...

full text

Molecular Detection of Salmonella Serovar Isolated from Eggs

Abstract Background and Objective: Salmonellosis is the most common type of food poisoning in developed and developing countries that is caused by Salmonella serotype. Hence, we aimed to identify the Salmonella serovars in eggs obtained from Kohgiluyeh and Boyerahmad province and to evaluate antibiotic resistance of the isolated strains. Material and Methods: In this study, 210 eggs were co...

full text

characterization of the salmonella isolates from backyard chickens in north of iran, by serotyping, multiplex pcr and antibiotic resistance analysis

the present study was designed to investigate the prevalence of salmonella species, their molecular characterization and antibiotic resistance in backyard chickens. a total of 1125 samples were collected from backyard chickens in two consequent samplings. in the first part, samples were included of 820 poor recently hatched chicks, hatching residuals, egg shells in the nest floor, cloacal swabs...

full text

My Resources

Save resource for easier access later


Journal title:
iranian journal of veterinary research

Publisher: shiraz university

ISSN 1728-1997

volume 16

issue 3 2015

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023